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In the case of the disease gene, the alternative alleles will be the normal allele and the disease allele, and they can be distinguished by looking for occurrences of the disease in a family tree or pedigree.
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In performing the lyophilization, the lyophilization conditions are controlled so that the cyclophosphamide is precipitated as a hydrated polymorph and then the process is completed under conditions which are carefully selected so that water bound to the cyclophosphamide as the monohydrate is not removed during the remainder of the lyophilization process.
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One strategy is oncolytic viruses, which selectively replicate in and kill tumor cells.
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